sabatini lander list (Addgene inc)
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sabatini lander list
Sabatini Lander List, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sabatini+lander+list/Mouse+Two+Plasmid+Activity-Optimized+CRISPR+Knockout+Library(Pooled+Library+%231000000096)/pmc12765214-353-70-72
Average 93 stars, based on 15 article reviews
Sabatini Lander List, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sabatini+lander+list/Mouse+Two+Plasmid+Activity-Optimized+CRISPR+Knockout+Library(Pooled+Library+%231000000096)/pmc12765214-353-70-72
Average 93 stars, based on 15 article reviews
sabatini lander list - by Bioz Stars,
2026-09
93/100 stars
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Polymerase Chain Reaction:Article Title: Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunity Article Snippet: We have previously described lentiviral backbones used perform Gfap promoter-driven genetic deletions in vivo 26 , which are derived from lentiCRISPRv2 (F. Zhang, Addgene plasmid 52961) and express Cas9 driven by the ABC 1 D gfa2 Gfap promoter 67 , whereas sgRNA is under U6 promoter control. .. To exchange 20 nucleotide-long sgRNA sequences to target different genes, we performed PCR-based restriction cloning using Phusion HF master mix (Thermo Fisher Scientific, F-548S) and the following primers: U6-PCR-F 5′-AAAGGCGCGCCGAGGGCCTATTT-3′, U6-PCR-R 5′-TTTTTTGGTCTCCCGGTGTTTCGTCCTTTCCAC-3′, crRNA-R 5′-GTTCCCTGCAGGAAAAAAGCACCGA-3′, crRNA-F 5′-AAAAAAGGTC TCTACCG(N) 20 GTTTTAGAGCTAGAAATAGCAAGTT-3′, where N 20 marks the sgRNA substitution site. sgRNA design was performed using a combination of tools: Synthego ( https://design.synthego.com// ), CRISPick (SpyoCas9, Hsu (2013) tracrRNA, https://portals.broadinstitute . org/gppx/crispick/public) and the Article Title: Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunity. Article Snippet: Camilo Faust Akl, Brian M. Andersen, Zhaorong Li, Federico Giovannoni, Martin Diebold, Liliana M. Sanmarco, Michael Kilian, Luca Fehrenbacher, Florian Pernin, Joseph M. Rone, Hong-Gyun Lee, Gavin Piester, Jessica E. Kenison, Joon-Hyuk Lee, Tomer Illouz, Carolina M. Polonio, Léna Srun, Jazmin Martinez, Elizabeth N. Chung, Anton Schüle, Agustin Plasencia, Lucinda Li, Kylynne Ferrara, Mercedes Lewandrowski, Craig A. Strathdee, Lorena Lerner, Christophe Quéva, Iain C. Clark, Benjamin Deneen, Judy Lieberman, David H. Sherr, Jack P. Antel, Michael A. Wheeler, Keith L. Ligon, E. Antonio Chiocca, Marco Prinz, David A. Reardon & Francisco J. Quintana1,12,16 ✉ Cloning:Article Title: Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunity Article Snippet: We have previously described lentiviral backbones used perform Gfap promoter-driven genetic deletions in vivo 26 , which are derived from lentiCRISPRv2 (F. Zhang, Addgene plasmid 52961) and express Cas9 driven by the ABC 1 D gfa2 Gfap promoter 67 , whereas sgRNA is under U6 promoter control. .. To exchange 20 nucleotide-long sgRNA sequences to target different genes, we performed PCR-based restriction cloning using Phusion HF master mix (Thermo Fisher Scientific, F-548S) and the following primers: U6-PCR-F 5′-AAAGGCGCGCCGAGGGCCTATTT-3′, U6-PCR-R 5′-TTTTTTGGTCTCCCGGTGTTTCGTCCTTTCCAC-3′, crRNA-R 5′-GTTCCCTGCAGGAAAAAAGCACCGA-3′, crRNA-F 5′-AAAAAAGGTC TCTACCG(N) 20 GTTTTAGAGCTAGAAATAGCAAGTT-3′, where N 20 marks the sgRNA substitution site. sgRNA design was performed using a combination of tools: Synthego ( https://design.synthego.com// ), CRISPick (SpyoCas9, Hsu (2013) tracrRNA, https://portals.broadinstitute . org/gppx/crispick/public) and the Article Title: Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunity. Article Snippet: Camilo Faust Akl, Brian M. Andersen, Zhaorong Li, Federico Giovannoni, Martin Diebold, Liliana M. Sanmarco, Michael Kilian, Luca Fehrenbacher, Florian Pernin, Joseph M. Rone, Hong-Gyun Lee, Gavin Piester, Jessica E. Kenison, Joon-Hyuk Lee, Tomer Illouz, Carolina M. Polonio, Léna Srun, Jazmin Martinez, Elizabeth N. Chung, Anton Schüle, Agustin Plasencia, Lucinda Li, Kylynne Ferrara, Mercedes Lewandrowski, Craig A. Strathdee, Lorena Lerner, Christophe Quéva, Iain C. Clark, Benjamin Deneen, Judy Lieberman, David H. Sherr, Jack P. Antel, Michael A. Wheeler, Keith L. Ligon, E. Antonio Chiocca, Marco Prinz, David A. Reardon & Francisco J. Quintana1,12,16 ✉ |